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・ Isotope 244
・ Isotope analysis
・ Isotope dilution
・ Isotope effect
・ Isotope electrochemistry
・ Isotope fractionation
・ Isotope geochemistry
・ Isotope hydrology
・ Isotope lists
・ Isotope lists, 0-24
・ Isotope lists, 25-48
・ Isotope lists, 49-72
・ Isotope lists, 73-96
・ Isotope lists, 97+
・ Isotope separation
Isotope-coded affinity tag
・ Isotope-ratio mass spectrometry
・ Isotopes of actinium
・ Isotopes of aluminium
・ Isotopes of americium
・ Isotopes of antimony
・ Isotopes of argon
・ Isotopes of arsenic
・ Isotopes of astatine
・ Isotopes of barium
・ Isotopes of berkelium
・ Isotopes of beryllium
・ Isotopes of bismuth
・ Isotopes of bohrium
・ Isotopes of boron


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Isotope-coded affinity tag : ウィキペディア英語版
Isotope-coded affinity tag
An Isotope-coded affinity tag (ICAT) is an isotopic labeling method used for quantitative proteomics by mass spectrometry that uses chemical labeling reagents.〔 "Rapid quantitative analysis of proteins or protein function in complex mixtures," Rudolf Hans Aebersold et al.〕 These chemical probes consist of three elements: a reactive group for labeling an amino acid side chain (e.g., iodoacetamide to modify cysteine residues), an isotopically coded linker, and a tag (e.g., biotin) for the affinity isolation of labeled proteins/peptides.
==Development==
The original tags were developed using deuterium, but later the same group redesigned the tags using 13C instead to circumvent issues of peak separation during liquid chromatography due to the deuterium interacting with the stationary phase of the column.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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