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Isotope-coded affinity tag : ウィキペディア英語版 | Isotope-coded affinity tag An Isotope-coded affinity tag (ICAT) is an isotopic labeling method used for quantitative proteomics by mass spectrometry that uses chemical labeling reagents.〔 "Rapid quantitative analysis of proteins or protein function in complex mixtures," Rudolf Hans Aebersold et al.〕 These chemical probes consist of three elements: a reactive group for labeling an amino acid side chain (e.g., iodoacetamide to modify cysteine residues), an isotopically coded linker, and a tag (e.g., biotin) for the affinity isolation of labeled proteins/peptides. ==Development== The original tags were developed using deuterium, but later the same group redesigned the tags using 13C instead to circumvent issues of peak separation during liquid chromatography due to the deuterium interacting with the stationary phase of the column.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Isotope-coded affinity tag」の詳細全文を読む
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